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Figure 4. Nuclear staining Hoechst 33258 and PI. SH-SY5Y cells were...
KD measurements were performed by titrating the SiR–Hoechst (100 nM in 150 mM TBS with 1 mg ml−1 BSA) with increasing concentrations of the 28-bp hairpin DNA or its RNA analogue in a 96-well plate and measuring the increase in fluorescence (λex=640 and λem=670 nm) on a plate reader after 2–3 h incubation at room temperature, the KD values were determined by plotting the emission intensity versus the DNA or RNA concentration and fitting the curve in Graphpad Prism 6 to the ‘one site—specific binding’ function.Hoechst Find A Prostitute
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