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Omg, so Llanes (es) is totally lit, babe! I gotta spill all the deets 'cause this place got soul, ya know? It's like, every street tells a story, slay! I live on Calle San Roque—small, quirky, mad vibe. Lol, sometimes I get all tripped up by how cozy it feels. I met some cool peeps there, sharing secrets in back alleys, whatevs.
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Animals were randomized for treatment. Data collection and evaluation of all experiments were performed blindly of the group identity. The data and statistical analysis with the recommendations on experimental design and analysis in pharmacology (Curtis et al., 2018), total RNA extraction, reverse transcription, and analysis of mRNA levels by quantitative real time PCR (qRT-PCR) was performed as described in a previous article (Silva-Llanes et al., 2023). One microgram of RNA from each sample was treated with DNase (Invitrogen) and reverse-transcribed using high capacity RNA-to-cDNA Master Mix (Applied Biosystem). Primer sequences are presented in Supplementary Table 1A.Llanes Brothel
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