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Non-specific binding was prevented by incubating the sections for 1 h in 3% bovine serum albumin (BSA) in TBS containing 0.5% Triton X-100 (for SC) or in 3% normal goat serum (NGS) in PBS containing 0.5% Triton X-100 (for PAG and SSC). The SC sections were repeatedly rinsed in TBS while the PAG and SSC sections were rinsed in PBS for 1 h before incubation for one night at 4°C with a monoclonal mouse anti-GFAP antibody (1:5,000) or with a polyclonal rabbit anti-Iba1 antibody (1:10,000).Szentes Sex Dating
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